The Somascan platform

Proteomic biomarker discovery and validation for clinical researchers

Most protein assays excel in one or two areas

The somascan® platform is fundamentally different

The somascan platform is fundamentally different

A single platform for discovery,
validation, and delivery

 

The SomaScan® Assay uses Slow Off-Rate Modified Aptamers (called SOMAmer® reagents) to provide 5,000 highly reproducible measurements of circulating proteins from a single sample of plasma, serum, or urine.

UNIQUE REAGENTS​


SOMAmer reagents are synthetic, ssDNA sequences with protein-like appendages that tightly bind a specific protein target

DEVELOPED BY SELEX​


Precise requirements for binding and durability are controlled via a directed evolution process called SELEX

FOR DISCOVERY, VALIDATION, & delivery


Unlike antibodies, SOMAmer reagents are eternally consistent, enabling unmatched reproducibility

How does the somascan assay work?

 
    1. SOMAmer reagents are synthesized with a fluorophore, photocleavable linker, and biotin
    2. The SOMAmer reagents bound to streptavidin beads are used to capture proteins from a biological sample
    3. Unbound proteins are washed away
    4. Bound proteins are tagged with biotin
    5. A UV light breaks the photocleavable linker
    6. Non-specific complexes dissociate
    7. Polyanionic competitors prevent rebinding of non-specific complexes
    8. Biotinylated proteins (and bound SOMAmer reagents) are captured
    9. SOMAmer reagents are isolated and fluorophores measured

SEMINAL
SOMASCAN PUBLICATION

Introduces the SomaScan Assay and demonstrates how chemically modified aptamers can be used to detect and discover biomarkers.

Read open access 

improving selex
with modified aptamers​

Describes how additional functional groups were used to increase the diversity of aptamers in the SELEX process, expanding to 3,000 targets.

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SOMAMER REAGENT

CRYSTAL STRUCTURE

Reports the crystal structure of a SOMAmer reagent binding PDGF-BB. Unlike traditional aptamers, the SOMAmer reagent utilizes hydrophobic interactions and mimics the natural receptor.​

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